Single-molecule fluorescence spectroscopy of protein interaction and dynamics
Rotation:
1st
2nd
3rd
Area:
Life Sciences
Sunday, October 17, 2021
You will recombinantly express and purify the transcription factor ComK, which is responsible for a stochastic phenotype switch in bacteria. After labeling DNA with fluorophores, you will use single-molecule FRET experiments to investigate the affinity of ComK for its promoter sequence.