DNA repair in embryonic stem cells

The integrity of genetic information is important in any cell type, but is even more critical in embryonic stem cells, where any mutations might propagate to all progeny cells throughout development. This is highlighted by the tendency of ES and iPS cells to create tumors, which poses a major obstacle to the safe use of stem cell-based regenerative medicine therapies. We are studying DNA repair in embryonic stem cells, with emphasis on DNA damage tolerance, and in particular TLS, which is of special interest because of its intrinsic mutagenicity. This work is done in collaboration with Dr. Lena Ainbinder (Stem Cell Core Unit). Methods include both plasmid-based and chromosomal TLS assays, along with genetic analysis using molecular, cellular and immunological techniques.