CryoSTEM tomography (CSTET) is an alternative to the better-know cryoTEM tomography, for which thickness limitations are several hundred nanometers.
We can probe 3D structures of cells up to around 1 micron in thickness.
This cryoSTEM tomogram shows T4 phage viruses attacking an E. coli bacterium.
The movie below shows a series of tilted cryoSTEM images of a human fibroblast cell
The movie below is stepping through the 3D volume that is reconstructed from the tilt series above. The scalebar is 400 nm.