Communication is very important for every living organism in order to survive, grow and proliferate. Cell-cell communication among unicellular organisms enables them to behave as a unified and coordinated community, rather than individually acting and is especially important for pathogens, as they are constantly facing the hostile environment of their host. Modes of communication include direct cell-to-cell contact and release of extracellular vesicles (EVs). EVs are commonly classified according to their mode of biogenesis (exosomes, 50-200nm in diameter and microvesicles 200-1000nm), and secreted both during homeostasis and pathogenic conditions. They mediate intercellular communication by fusing with distal cells and providing a secure and efficient mode for delivery of a wide range of bioactive components, including proteins, lipids, and nucleic acids. Due to their stability, exosomes protect their cargo against degradation and denaturation in the extracellular environment. Following their uptake, EVs can induce significant phenotypic changes in the recipient target cells. EVs play a crucial role in the complex life cycle of many parasites, including parasite growth and development, virulence factor transfer and evasion of immune responses. In this new area of malaria research, little is currently known about the precise mechanism of EV cargo loading, delivery and function. We aim to gain a better understanding on how malaria parasites sorts their cargo and releases it, as well as investigating its function as effector in the extracellular milieu and in the recipient target cells. The advanced-nano approaches developed in our lab not only yield significant information on one of the most lethal human pathogens, but will further lead to important advances in the field of EVs research more broadly.