Research

Cytokine signaling as a cascade of protein interactions for multiple outputs

How do similar ligands binding to the same receptor complex trigger diverse biological activities such as antiviral, antiproliferative, and immunomodulatory effects? To explore this phenomenon, we adopted a biophysical and cellular approach that delves into the detailed examination of different system components individually and within the complexity of the cellular environment.

Structure/function & evolution of protein interactions towards drug design

Protein complexation occurs through kinetic processes within the crowded cellular environment. We utilize biophysical tools, protein-engineering techniques, in vitro evolution, and simulations to deepen our understanding of protein interactions and to inform their design.

Targeted delivery of RNA using engineered exosomes

Our project tackles a fundamental obstacle impeding the clinical advancement of RNA therapies: their delivery. We are investigating extracellular vesicles (EVs) as an ideal carrier for biological macromolecules, such as coding RNA, due to their natural biocompatibility and potential for customization, given their origin from human cells. Our objective is to develop a flexible system for the efficient encapsulation of mRNA within EVs. We have already established a pioneering technique that allows cells to generate EVs tailored to engage with particular receptors on target cells. Advancing from this groundwork, we are now introducing innovative methods to actively load mRNA into EVs and direct them toward specific organs, with a focus on the lungs. This initiative promises to not only deepen our insight into RNA localization mechanisms but also to forge a targeted, EV-centric delivery method for RNA therapeutics, representing a considerable leap forward in the field of personalized medicine

Investigate the role of interferons in CLL and Melanoma in mouse models

Using transgenic mice, either harboring the human type I interferon receptors of knockout of them we investigated the effect of different type I interferons and different modes of application on specific disease models, particularly CLL and melanoma. Here, we found that interferons are survival factors for CLL, and that the disease is less sever in the background of interferon receptor knockout mice. Conversely, in melanoma systemic interferons had a negative influence on disease progression, while, localized interferon administration was successful in reducing disease burden through modulation of the immune response.