Cell Disrupter and Sonicator

Instrument Specification

The unit has two different instruments for cell membrane disruption. Cell lysis is the first step in purifying specific contents produced in cells.

  • TS Series Cell Disrupter is used to disrupt the membrane of cells by passing them through a narrow valve under high pressure. By controlling the pressure, it is possible to break different membranes in cells or different cell types. In this way you can separate different components of the cell (e.g., for E. Coli you can differentiate between Release of DNA, Cytoplasmic Protein, Inclusion Bodies, Membrane protein/material) or break bacterial, fungal, plant or animal cells. 
  • A Sonics Vibra-Cell VC 750 sonicator uses ultrasonic vibrations for disruption of cell membranes. The unit has several different probes, for diverse sample volume and sonication amplitudes: Stepped microtip, Tapered microtip, 13mm tip and Cup Horns.

Location

Perlman Chemical Sciences Building Room 12

Staff Contacts