Cryo lamella-on-grid preparation is a FIB-based approach for the preparation of cellular samples for subsequent study by cryo-electron tomography (ET)  and which is applicable to all kinds of cells grown or deposited directly on EM grids. With the ion beam incident under a shallow angle, material is removed from above and below the target structures. This approach leaves behind a thin (200–500 nm) lamella supported on both sides by the bulk ice.  The milled lamellae open up large windows into the interior of the cells.