Simultaneous measurement of excitation and inhibition
The aim of this research is to investigate the interplay between excitatory and inhibitory inputs in the cortex of the mouse. Current methods of intracellular recording, such as voltage and current clamp have been used to measure inhibitory and excitatory inputs in isolation. However, Both methods display a similar issue as they provide a weighted average of the inputs over many trials. This poses a problem since neuronal activity dynamically fluctuates as a function of time. We developed a new analytical framework for simultaneous measurements of both the excitatory and inhibitory neuronal inputs during a single trial under current clamp recording.