The freeze fracture technique is used as a complementary technique to direct imaging by cryo-TEM, especially for viscous systems, systems containing micron-scale objects as well as cells, tissues and microorganisms which are too thick for plunge freezing (roughly >5-10 µm.) Freeze fractured samples prepared by this device are observed in the cryo-SEM under cryogenic temperatures. The device includes two electron beam evaporation guns, one for carbon and one for platinum/carbon (or other metals). Carbon coating is used mostly for protecting the sample against beam damage when high currents and acceleration voltages are used during EDS analysis. A metal layer followed by a carbon layer coating (double layer coating) are applied for observation at high magnifications. A thick platinum layer is used for FIB milling in the FIB/SEM.