The FEI Tecnai G2 F20 S/TEM microscope is used for advanced biological EM imaging, chemical analysis, and 3D structural elucidation by TEM  and STEM tomography, including of cryo-preserved specimens.

Characteristics & Accessories

• Accelerating voltage: 120-200 kV.
• E-beam source, Schottky field emission source.
• Thermo Fisher cryobox anticontaminator and a variety of Gatan cryo-holders (Gatan 626-60, 626-70, 914).
• Point resolution 0.27 nm. STEM line resolution ~0.34 nm.
• FEI Low-Dose software.
• Tomography-grade Compustage.
• CryoTEM imaging using low-dose methods of soft materials, macromolecules and cryo-preserved tissues and cells.
•  TVIPS TemCam-XF416 retractable 16-megapixel CMOS camera.
• STEM and cryoSTEM tomography using a Fischione HAADF detector at the 35 mm port, and a Gatan BF/DF detector below the fluorescent screen.
• EL-Mul Opal segmented STEM detector (experimental).
• Tomography software: SerialEM.
• Norhof LN2 microdosing system #905 with a 12-liter Dewar allows for overnight runs of the microscope (at room temperature).