A Biacore S200 is an SPR-based instrument which can measure interactions between small molecules (>200Da) and their targets. This is achieved, as it is far more sensitive than the Biacore 8K, which we also have. It is the up-to-date development of Biacore line of products, by Cytiva. Biacore S200 raises the standard for SPR systems in fragment screening and lead optimization. It is the most suitable instrument for proteomics studies and drug discovery for high throughput screening (HTS) of small molecule drug candidates. With increased sensitivity, this system enables interaction analysis with greater precision and confidence than ever before. During research, development and production, these data give insights into protein functionality, elucidate disease mechanisms and play a key role in the critical decisions needed for efficient development and production of therapeutics.
The DSC PEAQ is a Differential Scanning Microcalorimeter. The DSC is a highly sensitive technology for studying molecules in solution.
The DSC is a powerful tool that enables characterization of the thermal stability of proteins and other biomolecules. It is a simple method that delivers the ‘Gold Standard’ in TM biosimilarity and batch-to-batch comparability assessment, as well as for the optimization of purification and manufacturing conditions. Our DSC system is simple to use, requiring little assay development, and no labelling or immobilization.
Nanosight is used for rapid, automated analysis of size distribution and concentration of all types of nanoparticles in aqueous solutions ranging from ~40nm to 1000nm in diameter. Fluorescent particles can be measured with four lasers.
Biacore™ 8K is a high-throughput, high-sensitivity Surface Plasmon Resonance (SPR) system that enables fast and efficient screening, characterization, process optimization, and quality control of binding of biomolecules and low molecular weight molecules.
This instrument has 16 flow-cell SPR system that efficiently delivers binding data with excellent quality and high capacity, in screening, characterization, process optimization, and quality control.
It has predefined methods with the application of relevant parameters. Biacore™ Insight Software is the complete instrument control and data evaluation software. 8k machine has a learning software, Biacore Intelligent Analysis™, to rapidly analyze large data sets.
ITC, Isothermal titration microcalorimetry, is the gold standard and an essential tool in drug discovery and the study of biomolecule interactions.
MicroCal ITC systems directly measure the heat released or absorbed during a biomolecular binding event. The result is a direct, label-free measurement of binding affinity and thermodynamics in a single experiment. They deliver comprehensive information for studying a wide variety of biomolecular interactions.
Measuring is direct, label-free and in solution of binding affinity and thermodynamics in a single experiment, enabling the accurate determination of binding constants (KD), reaction stoichiometry (N), enthalpy (ΔH) and entropy (ΔS).
This provides a complete thermodynamic profile of the molecular interaction.
Microscale Thermophoresis (MST) is a biophysical assay to quantify the interaction between in-solution molecules, such as proteins and biomolecules. The MST instrument detects the motion of fluorescent molecules (labelled or intrinsic) along a microscopic temperature gradient, which indicates changes in the molecular hydration shell, charge, or size upon binding.
Our unit has Micro-, Red and Label free, as well as Pico (Red and Blue)- MST instruments.
Differential Scanning Fluorimetry (DSF) Panta (Nanotemper) detects changes in the fluorescence emission of fluorescent amino acids upon unfolding using nanoDSF, an advanced Differential Scanning Fluorimetry technology. The instrument can be used to induce thermal unfolding of up to 48 samples and to determine thermal unfolding transition temperatures. Furthermore, the instrument is equipped to investigate chemical unfolding and the free energy of unfolding ΔG. No dyes or additives are required.
Prometheus Panta has three technologies for stability characterization:
Thermal stability: nanoDSF measures the thermal stability of proteins using their intrinsic fluorescence.
Back reflection: measures the turbidity of the sample, that is, it identifies the formation of large amorphous aggregates.
Dynamic Light Scattering (DLS): used to assess the size of particles in solution. This implies whether the protein samples are well-folded, or whether there are other contaminating species or aggregations.
The DynaPro® NanoStar® II (Wyatt technology) is a cuvette-based DLS instrument used for the analysis of sizes of proteins, gene therapy vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, liposomes, lipid and metallic nanoparticles, and more. To accommodate scarce and costly samples, specially designed quartz cuvettes afford robust, accurate measurements with as little as 2 µL of solution, while the disposable cuvettes provide comparable sensitivity with as little as 4 µL.
Field-flow fractionation (FFF) (Post Nova) can separate quantitatively particles over a wide size range. A field is applied to a suspension or solution pumped through a long and narrow channel, perpendicular to the direction of flow. The results in separation of the particles present in the fluid, depending on their differing mobilities under the force exerted by the field.
