Voltage imaging is an emerging technology that enables optical recording of membrane potentials and spiking activities of neurons at millisecond timescale. Light-sheet microscopy in behaving larval zebrafish holds the potential for expanding this technology to a brain-wide scale and elevating the level of understanding of neural computations during animal behaviors.
Based on the emergence of a bright, photostable voltage indicator in 2019, we have been developing optical, genetic and computational methods for advancing this technology in collaboration with other laboratories.
Related literature:
[1] Abdelfattah*, Kawashima* et al.
"Bright and photostable chemigenetic indicators for extended in vivo voltage imaging"
Science. 365 (6454): 699-704 (2019)
[2] Freeman et al.,
“Mapping brain activity at scale with cluster computing.”
Nature Methods. 11(9):941-50 (2014).
[3] Vladimirov et al.,
“Light-sheet functional imaging in fictively behaving zebrafish.”
Nature Methods. 11(9):883-4 (2014).