Breast carcinoma and neutrophils in lung: LCM

Imaging of CMTMR labeled breast carcinoma cells (red) and their surrounding neutrophils by light sheet microscopy of whole lungs
Imaging of CMTMR labeled breast carcinoma cells (red) and their surrounding neutrophils by light sheet microscopy of whole lungs

Endogenous neutrophils were labeled 5 mins before harvesting the lungs with Alexa 647-labeled anti-Ly6G mAb. Lungs were inflated with low melting temperature agarose, excised and cleared. Autofluorescent lung stromal cells are depicted in green. Bar= 100µm.