Melanoma cell in the process of extravasation through a lung vessel

3D image of lungs imaged by light sheet microscopy (LSM). Recipient mice were injected with 20,000 B16-F10 labeled with CMTMR 3 hours before. To visualize lung vessels, Alexa 647 conjugated anti CD31 mAb was injected 5 minutes before lungs were harvested, fixed and cleared for LSM imaging.