Research

Stem-Cell-Derived Embryo Models (SEMs)

Starting solely with naïve ESCs, we can unleash the cells ability to self-organize into embryoid models. This way we generated mouse SEM which is equivalent to embryonic day 8.5 (post-gastrulation/early organogenesis). Recently we have also generated human SEMs, which model 14 days-post-fertilization embryo. The model includes both embryonic and extra-embryonic (placental) tissues.

Ex Utero Embryogenesis: From Stem Cells to Organs

Our group has established a robust electronically controlled ex utero whole embryo culture platforms that allow capturing normal mouse embryogenesis from pre-gastrulation (E5.5) until advanced organogenesis (E11) ex utero.

Understanding Naïve and Primed Pluripotent States

Human stem cells can be maintained in a naive state, with distinct molecular and functional properties, including enhanced ability to contribute to cross-species mouse chimeric embryos. We found that Inhibition of SRC, PKC and WNT, consolidates human naive pluripotency induction.

Deciphering Cellular Reprogramming

We validate a distinct molecular axis, Gatad2a-Chd4-Mbd3, within Mbd3/NuRD as being critical for blocking reestablishment of naive pluripotency. Removal/Repression of components of this complex, brings to high cellular reprogramming efficiency.

Human-Mouse Cross-Species Chimerism

Human stem cells that are sustained in naïve culture conditions, can be injected to mouse blastocyst and contribute to cross-species chimera (Gafni et al, 2013). We investigate these chimeric mice, which are valuable tool for human disease modeling in a whole-organism context.