Single molecule Fluorescent In Situ Hybridization (smFISH)
Using the smFISH protocols, single RNA molecules can be detected and quantified in tissue sections,
whole mount samples and cell cultures.
smFISH illustration, courtesy of Binyamin Zuckerman, Ulitsky lab (also see relevant publication).
We offer:
- Assistance in designing probes
- Protocol calibration
- Troubleshooting
- Imaging
- Image analysis tools
smFISH experiments in our unit can be performed in one of the following methods:
- Using Stellaris RNA FISH probes
- Using the RNAscope kit
- RNAscope® Technology: A Novel RNA in situ Hybridization Research Platform
- RNAscope® Fluorescent Multiplex Assay
- RNAScope® Multiplex Fluorescent V2 Assay
- Using the HCR 3.0 kit